Journal: Fundamental Research

Article Title: Cancer-associated fibroblast-derived stanniocalcin 1 promotes cholangiocarcinoma progression and lymph node metastasis

doi: 10.1016/j.fmre.2023.11.022

Figure Lengend Snippet: CAF-derived STC1 was correlated with LNM in CCA. (a) Proteomics analysis volcano plot showing that STC1 was significantly upregulated in LN + CAF supernatant compared with LN − CAF supernatant. X-axis: the log2-fold change for each identified secretory protein from the supernatant. Y-axis: the −log10 of the p-values. Red dots indicate significantly upregulated proteins; blue dots indicate significantly downregulated proteins ( p < 0.05, fold change > 2 [log2 = 1]). Horizontal dotted line: original p = 0.05. Dots with fold change < 2 (log2 = 1) and/or p > 0.05 are in gray. Statistical tests were 2-sided. (b) Secreted STC1 concentration in LN + and LN − CAF supernatants. (c) Western blotting results of STC1 expression levels in LN + and LN − CAFs. (d) GEPIA database analysis of the relative STC1 mRNA expression in matched CCA tissues ( n = 36) and adjacent non-CCA tissues ( n = 9). (e) Representative images of STC1 immunohistochemical staining in human CCA and non-cancer tissues. (f) Secreted STC1 concentration in the serum of 20 patients with CCA and 20 non-cancer controls (Health). (g) Representative images of STC1 immunohistochemical and double-labeled immunofluorescent staining in LN + and LN − CCA tissues. (h) Representative images of STC1 immunohistochemical and double-labeled immunofluorescent staining in CCA + and CCA − LN tissues. All in vitro experiments were performed with at least three biological replicates. * p < 0.05; ** p < 0.01; *** p < 0.001; using 2-tailed t -test. Original magnification: ×200.

Article Snippet: Briefly, 1 μg total RNA was isolated from 200 mg/ml STC1-treated CAFs and controls that had been incubated with VAHTS mRNA capture beads (Vazyme), enriched for polyA+ RNA, and then RNA libraries were constructed.

Techniques: Derivative Assay, Concentration Assay, Western Blot, Expressing, Immunohistochemical staining, Staining, Labeling, In Vitro

Journal: Fundamental Research

Article Title: Cancer-associated fibroblast-derived stanniocalcin 1 promotes cholangiocarcinoma progression and lymph node metastasis

doi: 10.1016/j.fmre.2023.11.022

Figure Lengend Snippet: Clinical correlations between STC1, integrin αVβ3, p-FAK, and p-YAP in CCA tissues. (a) GEPIA database analysis of the relative mRNA expression of STC1, integrin, FAK, and YAP in matched CCA tissues ( n = 36) and adjacent non-cancer tissues ( n = 9). (b) STC1, integrin αVβ3, p-YAP, and p-FAK expression in human CCA specimens. Bars: 100 µm. (c) Pearson correlation coefficient analysis of STC1, integrin αVβ3, p-YAP, and p-FAK expression in patients with CCA ( n = 50) by immunohistochemistry. (d) Kaplan-Meier analysis of the OS data of patients with CCA stratified by STC1 level with integrin αVβ3, p-YAP, and p-FAK.

Article Snippet: Briefly, 1 μg total RNA was isolated from 200 mg/ml STC1-treated CAFs and controls that had been incubated with VAHTS mRNA capture beads (Vazyme), enriched for polyA+ RNA, and then RNA libraries were constructed.

Techniques: Expressing, Immunohistochemistry